Our potent and partially selective hA₁ adenosine receptor fluorescent antagonist shows higher affinity for hA₁ (K_i = 8.60 nM) compared to hA_2A, hA_2B and hA₃ adenosine receptors (K_i = 98.38 nM, 72.24 nM and 231.01 nM respectively in radioligand binding assays). It allows to perform cell visualization in fluorescence microscopy, confocal microscopy and high content system experiments. It is potentially suitable for other fluorescence-based assays.