Our potent PAN adenosine receptors fluorescent antagonist shows affinity for hA₁, hA_2A, hA_2B and hA₃ adenosine receptors (K_i = 20.9 nM, 171.0 nM, 44.7 nM and 95.2 nM respectively in radioligand binding assays). It allows to perform cell visualization in fluorescence microscopy, confocal microscopy and high content system experiments. It is potentially suitable for other fluorescence-based assays.