Our potent and selective hA₃ adenosine receptor fluorescent antagonist shows high affinity for hA₃ receptor and partial selectivity over the other receptor subtypes (K_i = 52.7 nM for hA₃ receptor in radioligand binding assay). It has been validated in Fluorescence Polarization binding assays as a valid alternative to radioligand binding assays. It allows to perform cell visualization in fluorescence microscopy, confocal microscopy and high content system experiments. It is potentially suitable for other fluorescence-based assays.

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https://pubs.acs.org/doi/10.1021/acsmedchemlett.1c00598

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