High-content screening (HCS), also known as high-content analysis  (HCA), is a largely used methodology in drug discovery to identify compounds interacting with specific targets in whole cells. This assay is especially suited for high throughput screening with multi-well plates.

In a classical competition binding assay, cells are incubated with a fixed concentration of the fluorescent ligand followed by the addition of growing concentrations of the compound of interest (competitor). Fluorescence intensity is measured at each concentration to obtain a sigmoidal curve, suitable for affinity determination.

High Content Screening

CELT-331 hCB2 Cannabinoid receptor fluorescent ligand is suitable to measure the affinity of compounds for the hCB2 receptor in HCS competition binding experiments using HCS. Shown is a representative example of the competition binding between CELT-331 and the CB2 reference compound GW405833.

HCS validation performed in Innopharma Laboratory (EU-OPENSCREEN network).

Celtarys Ligands validated for High Content Screening assays

Do you want more information?

If you want to know more about how to use fluorescent ligands to characterize receptors with pharmacological interest with this and other technologies, click on the button to download the article “Fluorescent ligands: A new method to label your GPCRs”

Download PDF

    Fill in this information and we will send you an email with the PDF

    0